Real Time Machine

Real Time Machine

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Real Time Machine

The Real Time System is a new, high specification, economically priced real time thermal cycler from DIDAC. The capacity of 48 accommodates a unique and economical 48-well plate. The well format mirrors a 384-well plate therefore allowing the use of 16-channel pipettes. The small plate cuts reagent costs in half whilst still producing a strong fluorescence signal. Minimizing the plate size also significantly improves thermal uniformity and greatly reduces run times.

Specification of Real Time Machine

Volume per well

Validated for 5 to 20 ul

Detection sensitivity

1 copy

Temp uniformity

+- 0.1°C

Temp accuracy

+- 0.15°C @ 60°C and 95°C

Temperature range

30 to 100 degC

Average ramp rate

5.5 degC sec

Thermal system

Hollow silver block peltier-based with conductive fluid

Block format

48-well block


48-well custom plates and optical adhesive seals

Optical system

Dual LED excitation (452 to 486 nm and 542 to 582 nm)


Allelic Discrimination and High Resolution Melt

Dimensions closed (WxDxH)

34.5cm x 31cm x 32cm (13.6 in x 12.2 in x 12.6 inches)

Dimensions open (WxDxH)

34.5cm x 31cm x 37cm (13.6 in x 12.2 in x 14.5 inches)